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Analysis on the key control points of nucleic acid detection in blood stations

Date:2021/01/06 14:03:53
A blood station is a medical and health institution that collects and stores blood and supplies blood to clinical or blood product production units. With the rapid development of the medical industry, the management of clinical blood donation and blood products is becoming more and more standardized. The quality inspection of the blood station is the core of the management of the blood station, and the qualified importation of blood products is related to the life safety of thousands of families. At present, common clinical blood screening methods are serological testing and nucleic acid testing, which can detect whether blood donors are infected with AIDS, syphilis, hepatitis B and other pathogens to ensure the safety of blood products. This article discusses the application of nucleic acid testing in blood stations. Nucleic acid testing can directly detect the pathogen's genome, improve the efficiency of blood testing, and reduce the spread of pathogens. Carrying out blood station nucleic acid testing is a fund-control measure to ensure blood safety. In the blood station nucleic acid testing, the laboratory management is fully done, and the strict aseptic operation can ensure the precision of testing, continuously improve the quality of blood products, and ensure clinical It is safe to use blood in the future.
With the rapid development of the cause of unpaid blood donation, more and more people donate blood. Blood transfusion is one of the important means to rescue and treat critically ill patients. The number of clinical blood donors is increasing year by year. To ensure the integrity of blood quality, blood testing is very important. [1]. The blood station divides the blood collection process and management into many details according to relevant regulations, and requires strict management of each link to ensure the quality of blood products. The station has completed 300,000 nucleic acid sample tests since May 2014. Now, combining actual work, I will talk about the key control points that the blood station nucleic acid testing laboratory needs to pay attention to.
1 Division of each area of the nucleic acid laboratory
To ensure the accuracy and reliability of nucleic acid testing results, one of the important points is pollution prevention. Therefore, the laboratory area must be reasonably divided in strict accordance with the requirements of nucleic acid laboratory specifications. At present, the station has established two nucleic acid testing laboratories, which are divided into three areas according to requirements and work needs: reagent storage area (area 1), sample processing area (area 2), and amplification area (area 3). Each area is completely independent and cannot be connected; each area has an independent ventilation system: the pressure in the first to the third area decreases successively, the first and second areas maintain positive or normal pressure, and the three areas maintain negative pressure. The two nucleic acid laboratories are equipped with two nucleic acid detection systems from Haoyuan and Huayimei, which can back up each other to deal with emergencies.
The staff should check the pressure in each zone before the experiment, keep the first zone> zone two> zone three, zone one and zone two keep positive pressure or normal pressure, zone three are in negative pressure state. Inspectors and articles flow in one direction from zone one to zone two to zone three, irreversibly. Buffer rooms are set up in each area, where work clothes and work shoes are placed, and are dedicated to the area. Work clothes and work shoes cannot be worn outside the area. All items in the laboratory are dedicated and labeled with each area to prevent mixed use.  2 Management of nucleic acid laboratory operators
Nucleic acid detection is an emerging molecular biology technology, which has strict requirements on inspectors. Nucleic acid laboratory pre-inspectors should undergo pre-job training, enter the laboratory after obtaining the consent of the head of the department, and receive training on the use of reagents and equipment from the manufacturer. They can operate independently and pass the assessment before entering the laboratory. And regularly organize laboratory function personnel to conduct business schools or advanced studies, and regularly require each staff member to give PPT lectures. The content of the lectures is the content of the laboratory work. We will discuss the specific problems encountered in daily work, and we will solve them together to improve the quality of work. .
3 Quality Management System of Nucleic Acid Testing Laboratory
In order to ensure the safety of blood recipients and the safety of inspectors, a quality management system for the nucleic acid room has been established, which includes management systems, job responsibilities, and standard operating procedures.
①The management system includes the anti-pollution and biological safety system of nucleic acid laboratory, the access system and the management system of nucleic acid laboratory; each class of staff records the tasks of the class, so that they can be documented and verified.
②The job responsibilities include the receipt, preservation and processing of specimens, nucleic acid testing of specimens, the issuance of inspection reports, indoor quality control and inter-room quality evaluation, and notification of positive test results.
4 Nucleic acid detection operation process
The quality of nucleic acid test specimens directly affects the accuracy of the test results. Therefore, sterile, DNase-free, RNase-free, and vacuum blood collection tubes with separation gel containing citrate or EDTA anticoagulant should be used. The remaining sample size is 5 mL. After collection, it should be slowly and gently inverted and mixed for 5-8 times immediately, centrifuged in a low-temperature centrifuge within 4 hours, and then stored in a refrigerator at 2-8℃. If the person receiving the specimen finds that the specimen has severe hemolysis, lipemia, low volume, or unchecked specimen information, they should refuse to accept it. The specimens should be tested within 72 hours. If it cannot be completed, the specimens should be frozen below -20°C. The specimens should be re-thawed in a refrigerator at 2-8°C or at room temperature before being taken out again for testing.

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